Q5 Tm Calculator

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New England Biolabs - Official Site

    The NEB Tm calculator is designed to recommend optimal annealing temperatures for primer pairs. To get Tm values for a list of single primers, enter them one per line (ID1; Sequence1). The software will process the line as having an invalid second primer and will not calculate a Ta, but will calculate the Tm of the first p… See more

Q5® High-Fidelity DNA Polymerase | NEB

    Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended. …

Q5® Hot Start High-Fidelity DNA Polymerase | NEB

    Q5 Hot Start High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB T m Calculator is highly recommended. …

Molecular Biology Research Tools | NEB

    Product Selection. Competitor Cross-Reference Tools. Use this tool to select another …

NEB Tm Calculator – LabTools

    The tool fields are: Product Group: select a value from Q5, Q5 Hot Start, Q5U Hot Start, OneTaq, OneTaq Hot Start, Hot Start Taq, Taq DNA Polymerase, LongAmp Taq, LongAmp Hot Start Taq, Hemo KlenTaq, …

Tm Calculator | Thermo Fisher Scientific - US

    How to use the Tm calculator. The calculator calculates recommended T m (melting temperature) of primers and PCR annealing temperature based on the primer pair …

PCR Troubleshooting Guide | NEB

    Recalculate primer Tm values using the NEB Tm calculator; Mispriming: Verify that primers have no additional complementary regions within the template DNA; …


    New users should follow the Quick-Start Steps list on the left to get started. Hover over each step for additional information. Details on NEBaseChanger and the Q5 Site-Directed Mutagenesis Kit (E0554) can be accessed via …

Q5/Phusion PCR - Bennett Lab Wiki - Rice University …

    Q5 is popular because it is characterized to be the highest-fidelity PCR polymerase engineered thus far (1), with a fidelity 280-fold that of Taq polymerase. The …

QuickChange Primer Design - Agilent Technologies

    The QuikChange® Primer Design Program supports mutagenic primer design for your QuikChange mutagenesis experiments. Using primer design guidelines described in …

Q5 Tm Calculator & other calculators

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