Ligation Calculator

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Ligation Calculator - NEB

    https://nebiocalculator.neb.com/#!/ligation
    Ligation Calculator This tool will calculate the mass of insert required at several molar insert:vector ratios in the range needed for typical ligation reactions. Ligation Tutorials Insert DNA length Vector DNA length Vector DNA mass Required insert DNA mass --- (1:1) --- (2:1) --- (3:1) --- (5:1) --- (7:1) Formula

LIGATION CALCULATOR - HHU

    http://www.insilico.uni-duesseldorf.de/Lig_Input.html
    LIGATION CALCULATOR LIGATION CALCULATOR Please provide the following information: ......vector size (in bp): vector amount (in ng): ......insert size (in bp): Please …

Ligation Calculator - MDApp

    https://www.mdapp.co/ligation-calculator-545/
    Ligation Calculator Determines the optimal insert amount for a cloning reaction based on vector and insert properties. Key Facts Vector size Vector amount …

Ligation Calculator - Gene Link

    https://www.genelink.com/tools/gl-lc.asp
    Ligation Calculator. PART 1 : Convert double strand DNA (ds DNA) to pmoles and ends. DNA Name. ds DNA size in base pairs (bp) Quantity in micogram (ug) PART 2 : Ligation …

Addgene: Protocol - How to Ligate Plasmid DNA

    https://www.addgene.org/protocols/dna-ligation/
    Use a ligation calculator to easily quantify how much vector and insert DNA to use. Combine the following in a PCR or Eppendorf tube: Vector DNA Insert DNA Ligase Buffer (1μL/10μL reaction for 10X buffer, and …

Molecular Biology Research Tools | NEB

    https://www.neb.com/tools-and-resources/interactive-tools
    Calculators NEBioCalculator® Use this tool for your scientific calculations and conversions for DNA and RNA. Options include conversion of mass to moles, ligation amounts, …

Early Career Researcher Toolbox: Free Online Molecular …

    https://blog.addgene.org/free-online-molecular-biology-tools
    NEB Ligation Calculator: This tool works for any cloning method. You can select from five different insert:backbone ratios. In-Fusion cloning molar ratio calculator Lab math calculators These calculators can help you with …

Ligation Calculator - PrecisionMDApp

    https://precisionmdapp.com/ligation-calculator-545/
    Ligation Calculator Determines the optimal insert amount for a cloning reaction based on vector and insert properties. Purpose Key Facts Contents Vector size Vector amount …

Ligation Protocol with T4 DNA Ligase (M0202) | NEB

    https://www.neb.com/protocols/0001/01/01/dna-ligation-with-t4-dna-ligase-m0202
    NEBioCalculator ® - Using the Ligation module This tutorial describes the use of the NEBioCalculator web tool to optimize the molar ratio between vector and insert DNA for …

Optimize Your DNA Ligations - Thermo Fisher Scientific

    https://www.thermofisher.com/us/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/optimize-dna-ligase.html
    The equation below can be used to calculate the even molar ratio in nanograms of insert DNA to vector DNA based on length: length of insert (bp) x ng of vector = ng of insert …

Ligation Calculator & other calculators

Online calculators are a convenient and versatile tool for performing complex mathematical calculations without the need for physical calculators or specialized software. With just a few clicks, users can access a wide range of online calculators that can perform calculations in a variety of fields, including finance, physics, chemistry, and engineering. These calculators are often designed with user-friendly interfaces that are easy to use and provide clear and concise results.